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In Vivo Sublayer Analysis of Human Retinal Inner Plexiform Layer Obtained by Visible-Light Optical Coherence

Zeinab Ghassabi¹, Roman V Kuranov^2,3, Joel S Schuman^1,4,5,6,7,8

¹Department of Ophthalmology, NYU Langone Health, NYU Grossman School of Medicine, New York, New York, United States.

Investigative Ophthalmology & Visual Science

|January 13, 2022

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Visible-light optical coherence tomography (vis-OCT) can detect thinning in the inner plexiform layer (IPL) of glaucoma patients. This technology offers a repeatable method for assessing IPL sublayer changes in glaucoma, aiding early detection.

Area of Science:

Ophthalmology
Medical Imaging
Neuroscience

Background:

Glaucoma is a leading cause of irreversible blindness.
Retinal ganglion cell (RGC) dendrite degeneration may precede RGC death in glaucoma.
Understanding inner plexiform layer (IPL) structural changes is crucial for glaucoma diagnosis.

Purpose of the Study:

To investigate the capability of visible-light optical coherence tomography (vis-OCT) in detecting differences in IPL sublayer thicknesses between healthy and glaucomatous subjects.
To assess the repeatability of vis-OCT for IPL sublayer thickness measurements in glaucoma patients and healthy individuals.

Main Methods:

Utilized vis-OCT to image the retinas of nine healthy and five glaucomatous subjects.
Performed manual segmentation of IPL sublayers using averaged A-line profiles.

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Assessed intrasession repeatability using median coefficients of variation (CVs).

Main Results:

Glaucoma patients were significantly older (P = 0.02) and had significantly worse visual field mean deviations (P = 0.002) than healthy subjects.
Mean IPL thickness was significantly reduced in glaucomatous eyes (36.2 ± 1.5 µm) compared to healthy eyes (40.1 ± 1.7 µm) (P = 0.003).
Intrasession repeatability (CVs) for the entire IPL and sublayers was good in both groups, with some variation between sublayers.

Conclusions:

The middle IPL sublayer may account for the majority of IPL thinning observed in glaucoma.
Vis-OCT is a repeatable tool for quantifying IPL sublayer thicknesses in both healthy and glaucomatous eyes.
These findings support the potential of vis-OCT for advancing glaucoma clinical management and understanding.