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Related Concept Videos

Adhesion01:14

Adhesion

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Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
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Selectins01:25

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Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
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Catenins01:23

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Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
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Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
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Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion
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Comparison between Catechol- and Thiol-Based Adhesion Using Elastin-like Polypeptides.

Charng-Yu Lin1, Julie C Liu1,2

  • 1Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN 47907, United States.

ACS Applied Bio Materials
|January 13, 2022
PubMed
Summary
This summary is machine-generated.

Marine mussel proteins inspire wet adhesion strategies. This study compares DOPA and thiol chemistries in elastin-like polypeptides, finding thiol offers faster curing and stronger adhesion, while DOPA excels in recovery, with combined use yielding optimal results.

Keywords:
DOPAbiomedical adhesiveelastin-like polypeptidemussel adhesive proteinsrecombinant proteinthiol−catechol adduct

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Surface Chemistry

Background:

  • Marine mussel adhesive proteins utilize l-3,4-dihydroxyphenylalanine (DOPA) and thiol-rich proteins for wet adhesion.
  • DOPA's multiple reaction mechanisms and thiols' disulfide bond formation contribute to adhesion in humid conditions.
  • Understanding the distinct and synergistic roles of these chemistries is crucial for developing advanced wet adhesives.

Purpose of the Study:

  • To investigate the individual and combined contributions of DOPA and thiol chemistries to adhesive properties.
  • To compare the curing kinetics, disturbance recovery, and cytocompatibility of DOPA- and thiol-based model protein adhesives.
  • To identify optimal strategies for designing high-performance adhesives for humid environments.

Main Methods:

  • Engineered elastin-like polypeptides (ELPs) rich in either DOPA or thiol residues, maintaining high sequence similarity.
  • Comparative analysis of curing kinetics under wet conditions for DOPA- and thiol-based ELPs.
  • Evaluation of adhesive performance, including strength and recovery from disturbances, and assessment of cytocompatibility.

Main Results:

  • Both DOPA and thiol chemistries yielded cytocompatible adhesives.
  • Thiol-based adhesives exhibited faster curing kinetics and superior adhesion strengths.
  • DOPA-based adhesives demonstrated enhanced recovery capabilities after disturbances during curing.
  • Simultaneous use of DOPA and thiol chemistries with iron ions achieved a balance of fast curing, strong adhesion, and good recovery.

Conclusions:

  • DOPA and thiol chemistries offer distinct advantages for wet adhesion, with thiol excelling in speed and strength, and DOPA in resilience.
  • Combining DOPA and thiol chemistries, particularly with iron ions, provides a synergistic approach to optimize adhesive performance in humid environments.
  • These findings offer valuable insights for the rational design of next-generation wet adhesives.