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Published on: March 30, 2014
Multicentre interlaboratory analysis of routine susceptibility testing with a challenge panel of resistant strains
Corentin Deckers1, Reza Soleimani1, Olivier Denis1
1Laboratory of Microbiology, CHU UCL Namur and Antibiotic-Resistant Gram-Negative Bacilli National Reference Center, Yvoir, Belgium.
Objectives:
In order to elaborate a new national challenge panel of resistant Gram-negative bacilli and Gram-positive cocci strains for the validation of routine antimicrobial susceptibility testing (AST) methods, an interlaboratory evaluation was organised.
Methods:
The results of 12 well-characterised multidrug-resistant strains tested by nine laboratories using local disk diffusion (DD) and automated AST (AUST) methods were compared with the reference broth microdilution method.
Results:
Overall categorical agreement ranged from 70% to 100% both for DD and AUST and was >90% for all but one strain for all antibiotics.
Conclusion:
Our multicentre AST study showed good reproducibility and the panel can be used as national resistant reference strains for routine AST validation.
