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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
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Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system.

Monireh Bakhshpour1, Elisa Chiodi2, Iris Celebi2

  • 1Hacettepe University, Department of Chemistry, Ankara, Turkey; Boston University, Department of Electrical and Computer Engineering, Boston, MA, United States.

Biosensors & Bioelectronics
|January 13, 2022
PubMed
Summary

This study introduces a new Interferometric Reflectance Imaging Sensor (IRIS) for label-free detection of immunoglobulin G (IgG). The sensor offers highly sensitive, real-time quantification of IgG in aqueous solutions for potential clinical applications.

Keywords:
Immunoglobulin GInterferometric reflecting imaging sensorMedical applicationsReal-time detection

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Area of Science:

  • Biomedical Engineering
  • Biosensing Technology
  • Analytical Chemistry

Background:

  • Traditional biomolecular detection methods have limitations.
  • There is a need for label-free, sensitive, real-time, and cost-effective sensors.
  • Innovative sensor development is crucial for advancing diagnostics.

Purpose of the Study:

  • To demonstrate immunoglobulin G (IgG) detection using the Interferometric Reflectance Imaging Sensor (IRIS) system.
  • To evaluate the sensor's performance for label-free, real-time kinetic measurements.
  • To explore the potential of IRIS for direct biomarker detection in clinical applications.

Main Methods:

  • Utilized the Interferometric Reflectance Imaging Sensor (IRIS) system for detection.
  • Performed real-time, label-free kinetic measurements.
  • Conducted kinetic characterization experiments to evaluate sensor performance.

Main Results:

  • Achieved a high correlation coefficient (>0.94) for IgG detection.
  • Established a low detection limit of 0.25 μg/mL (1.67 nM) for IgG.
  • Demonstrated accurate quantification and reliable validation of binding events.

Conclusions:

  • The IRIS system provides a highly sensitive and reliable method for IgG detection in aqueous solutions.
  • The sensor's capabilities offer new perspectives for direct biomarker detection in clinical settings.
  • Label-free, real-time kinetic measurements with IRIS show promise for advancing diagnostic tools.