Development of magnetic solid phase extraction using magnetic amphiphilic polymer for sensitive analysis of...

A sensitive and time-saving method for the determination of multi-pesticide residues in honey was developed using magnetic solid phase extraction (MSPE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Magnetic polymer (N-vinyl pyrrolidone-divinyl benzene) (MVP-DB) was fabricated and employed as the adsorbent for extraction and enrichment of multi-pesticide residues in honey. MVP-DB contains lipophilic benzene ring, divinyl group, and hydrophilic pyrrolidone group. The good hydrophilic and hydrophobic structure of MVP-DB not only ensures sufficient dispersion in honey samples, but also enhances the ability to enrich target analytes. The predominant factors affecting the recoveries of analytes were systematically investigated, affording a rapid and highly efficient MSPE method. Under the optimal conditions, the method was verified, including the recovery, precision, linearity, sensitivity, and matrix effects. The results displayed that these pesticides showed good linearity in the range of 2-250 µg L^-1. The MLOQs were 0.5 µg kg^-1. The recoveries of pesticides in honey at the pre-spiked concentrations of 0.5-25 µg kg^-1 were 61.6%-112% with RSDs less than 18.2%. Hence, the developed method displayed good application prospect for the determination of multi-pesticide residues in honey samples.