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Highly-sensitive and simple fluorescent aptasensor for 17 b-estradiol detection coupled with HCR-HRP structure
1State Key Laboratory of Analytical Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, No 163, Xianlin Avenue, Nanjing, 210023, China.
Talanta
|January 13, 2022
Summary
This study presents a novel fluorescent aptasensor for detecting 17β-estradiol (E2), an environmental endocrine disruptor. The method achieves highly sensitive detection of E2 in real samples, offering a new approach for contaminant monitoring.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Biotechnology
Background:
- 17β-estradiol (E2) is a significant environmental endocrine disruptor affecting human growth and reproduction.
- The widespread abuse of E2 in breeding necessitates sensitive detection methods for environmental monitoring.
Purpose of the Study:
- To develop a highly sensitive and simple fluorescent aptasensor for detecting low concentrations of E2.
- To utilize hybridization chain reaction (HCR) and horseradish peroxidase (HRP) for signal amplification.
Main Methods:
- Aptasensor construction involving magnetic beads, complementary DNA (cmDNA), and polystyrene microspheres.
- Signal amplification via HCR-induced biotinylation and streptavidin-horseradish peroxidase (SA-HRP) catalysis.
- Fluorescence detection for quantifying E2 concentrations.
Main Results:
- The aptasensor demonstrated a linear detection range of 1-100 pg/mL for E2.
- A low detection limit of 0.2 pg/mL was achieved, surpassing previous methods.
- High recovery rates (91.07%-108.06%) were observed in real milk and water samples.
Conclusions:
- The developed fluorescent aptasensor offers a sensitive and effective method for E2 detection.
- This assay provides a promising approach for monitoring various environmental contaminants in real-world samples.
