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Updated: Jul 17, 2026
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Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
Published on: March 13, 2013
Cycle reset in a melanoma cell line caused by cooling
1Cancer Research Campaign Gray Laboratory, Mount Vernon Hospital, Middlesex, U.K.
Cell and Tissue Kinetics
|November 1, 1987
Summary
Non-standard temperatures during cell cycle G0-G1 transition cause delays in DNA synthesis. Lower temperatures (8°C, 25°C) impact cell cycle progression and S phase entry, suggesting temperature-sensitive regulatory mechanisms.
Area of Science:
- Cell Biology
- Molecular Biology
- Biophysics
Background:
- Cells require specific conditions to re-enter the cell cycle after G0 arrest.
- Temperature is a critical environmental factor influencing cellular processes.
Purpose of the Study:
- To investigate the impact of non-standard temperatures on cell cycle re-entry and DNA synthesis.
- To understand how temperature affects the G0-G1 transition in mouse melanoma cells.
Main Methods:
- Utilized a mouse melanoma cell line (HP2).
- Applied cold pulses (8°C and 25°C) during the G0-G1 transition.
- Measured DNA synthesis initiation and S phase progression using pulse-thymidine incorporation.
Main Results:
- Cold exposure (8°C) induced a delay in S phase entry proportional to the cold exposure duration.
- Cooling to 25°C caused a variable delay, dependent on the timing within G0-G1.
- Later cooling stages in G0-G1 resulted in a higher S phase peak height, independent of total incubation time.
Conclusions:
- A labile substance, crucial for cell cycle progression, is differentially degraded at 25°C compared to 8°C.
- Temperature-sensitive events regulate both the timing of S phase entry and the overall rate of DNA synthesis.
