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Related Concept Videos

Ion Exchange01:17

Ion Exchange

691
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
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Extraction: Advanced Methods00:56

Extraction: Advanced Methods

564
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
564
Colloidal precipitates01:09

Colloidal precipitates

839
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
839

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A method for purifying nanoparticles using cationic modified monoliths and aqueous elution.

Masaru Kato1, Misa Yamaguchi1, Tomoka Morita1

  • 1Department of Bioanalytical Chemistry, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

Journal of Chromatography. A
|January 14, 2022
PubMed
Summary

This study introduces novel silica monoliths for purifying nanoparticles, crucial for safe medical applications like vaccines and drug delivery. The method efficiently separates nanoparticles from contaminants using simple centrifugation.

Keywords:
Aqueous solutionCationic modified monolithExosomeNanomedicinePurification

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Nanoparticles are increasingly vital in medical diagnostics and therapeutics, particularly in vaccine delivery systems.
  • Ensuring the safety and purity of nanoparticles is paramount due to potential side effects.
  • Existing purification methods can be complex and time-consuming.

Purpose of the Study:

  • To develop and evaluate cationic modified silica monoliths for efficient nanoparticle purification.
  • To assess the separation capabilities of these monoliths for nanomedicines and exosomes from coexisting contaminants.
  • To establish a rapid and effective purification protocol for enhancing nanoparticle safety.

Main Methods:

  • Three types of cationic modified silica monoliths (NH₂, poly-Lys, trimethylaminopropyl) were synthesized.
  • Doxil (anticancer nanomedicine) and exosomes were purified from model solutions containing leaked drugs and proteins.
  • Purification was achieved using low-speed centrifugation and stepwise elution with varying Tris buffer concentrations and pH.

Main Results:

  • NH₂- and poly-Lys-modified monoliths successfully separated nanoparticles from leaked drugs and proteins.
  • Specific elution conditions were determined for Doxil (500-1000 mM Tris, pH 8 for NH₂; 200-1000 mM Tris, pH 6 for poly-Lys) and exosomes (1000 mM Tris, pH 8 for NH₂).
  • Recovery efficiencies for Doxil and exosomes were 64% and 55%, respectively.

Conclusions:

  • Cationic modified silica monoliths offer a rapid and efficient method for nanoparticle purification.
  • The technique utilizes simple, low-speed centrifugation, making it practical for various applications.
  • This purification approach holds significant potential for improving the safety and reliability of nanoparticle-based medical treatments.