Dual Engineering Interface-Driven Complementary Graphene Oxide-Protein Dimer Supramolecular Architecture Enables...

Area of Science:

• Biotechnology
• Nanotechnology
• Materials Science

Background:

• Developing versatile nanoplatforms for multimodal nucleus imaging and therapy remains a significant challenge in nanomedicine.
• Bottom-up bionanotechnology offers a promising approach for constructing complex supramolecular architectures with controlled self-assembly.

Purpose of the Study:

• To propose a general complementary bottom-up bionanotechnology for controlling 3D supramolecular coassembly.
• To demonstrate a dual engineering interface for a supramolecular architecture using genetically engineered protein dimers and plasmonically engineered graphene oxide (GO).

Main Methods:

• Utilized a genetically engineered protein dimer and plasmonically engineered graphene oxide (GO) to create a 3D supramolecular architecture.
• Incorporated anisotropic plasmonic nanoparticles as an intercalation layer within the GO architecture.
• Engineered specific two-site mutations in the plasmid to achieve organized coassembly of GO.

Main Results:

• Achieved tunable optical properties of GO across the ultraviolet-to-near-infrared region.
• Demonstrated continuous, distinguishable enhanced Raman imaging for tracking cancer cells.
• Showcased excellent nucleus therapeutic potential of the submicro 3D supramolecular coassembly in cancer cells.

Conclusions:

• The proposed bionanotechnology approach enables the controlled coassembly of GO-based nanostructures.
• The developed nanoplatform facilitates multimodal nucleus imaging and demonstrates significant therapeutic potential for cancer treatment.