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Updated: Oct 6, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

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Long noncoding RNA ADIRF antisense RNA 1 upregulates insulin receptor substrate 1 to decrease the aggressiveness of

Lingling Xu1, Yinling Tan2, Fengxia Xu2

  • 1Department of Oncology, Weifang Yidu Central Hospital, Weifang, Shandong China.

Bioengineered
|January 15, 2022
PubMed

Insights

Long noncoding RNA ADIRF-AS1 promotes osteosarcoma (OS) growth by sponging miR-761, leading to increased IRS1. Lowering ADIRF-AS1 expression inhibits OS progression and may offer new therapeutic strategies.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) play crucial roles in regulating gene expression.
  • The specific functions of adipogenesis regulatory factor-antisense RNA 1 (ADIRF-AS1) in osteosarcoma (OS) remain largely uncharacterized.
  • Understanding lncRNA involvement in OS is vital for developing novel therapeutic approaches.

Purpose of the Study:

  • To investigate the expression and clinical significance of ADIRF-AS1 in osteosarcoma.
  • To elucidate the functional role of ADIRF-AS1 in OS cell progression.
  • To explore the underlying molecular mechanism of ADIRF-AS1 in OS.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to detect ADIRF-AS1 expression levels.
  • Cell proliferation, migration, invasion, and apoptosis assays.
  • In vivo tumor growth experiments in nude mice.
  • RNA immunoprecipitation (RIP) and dual-luciferase reporter assays to confirm molecular interactions.

Main Results:

  • ADIRF-AS1 was significantly overexpressed in osteosarcoma tissues and associated with shorter patient survival.
  • Knockdown of ADIRF-AS1 suppressed OS cell proliferation, migration, and invasion, while promoting apoptosis.
  • ADIRF-AS1 functions as a competing endogenous RNA (ceRNA) by sponging miR-761, leading to the upregulation of its target gene, insulin receptor substrate 1 (IRS1).
  • Silencing miR-761 or restoring IRS1 expression could reverse the inhibitory effects of ADIRF-AS1 knockdown.

Conclusions:

  • ADIRF-AS1 promotes osteosarcoma oncogenicity by regulating the miR-761/IRS1 axis.
  • ADIRF-AS1 serves as a potential diagnostic biomarker and therapeutic target for osteosarcoma.
  • Targeting the ADIRF-AS1/miR-761/IRS1 pathway may represent a promising strategy for osteosarcoma treatment.
Keywords:
Microrna-761adirf-as1ceRNA theoryirs1

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