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Updated: Oct 6, 2025

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
Yuji Kohno1, Akiho Nakamura1, Shigeyuki Morishita1
1EM Research and Development Department 2, JEOL Ltd., 3-1-2, Musashino, Akishima, Tokyo 196-8558, Japan.
Abstract:
Differential phase contrast (DPC) scanning transmission electron microscopy can directly visualize electromagnetic fields inside a specimen. However, their image contrast is not only sensitive to the electromagnetic fields in the sample, but also the changes in diffraction conditions such as sample bends or thickness changes. These additional contrasts are called diffraction contrasts, and sometimes make it difficult to extract pure electromagnetic field information from the experimental DPC images. In this study, we developed a beam scan system that can acquire many DPC images from the same sample region with arbitrarily varying incident beam tilt angles to the sample. Then, these images are precisely averaged to form tilt-scan averaged DPC images. It is shown that the diffraction contrast can be effectively reduced in the tilt-scan averaged DPC images.
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