Down-regulation of MEG3, PANDA and CASC2 as p53-related lncRNAs in breast cancer

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Published on: March 27, 2020

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Soudeh Ghafouri-Fard1, Behnoush Sohrabi2, Bashdar Mahmud Hussen3

  • 1Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Breast Disease
|January 17, 2022
PubMed

Insights

Reduced expression of TP53 and related long non-coding RNAs (lncRNAs) like MEG3, CASC2, and PANDA is linked to breast cancer development. CASC2 shows potential as a diagnostic biomarker for breast cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • TP53 is a critical tumor suppressor gene in breast tissue.
  • Long non-coding RNAs (lncRNAs), including PANDA, MEG3, and CASC2, regulate TP53's expression and function.
  • Dysregulation of these molecules is implicated in various cancers.

Purpose of the Study:

  • To investigate the expression levels of TP53 and specific lncRNAs (PANDA, MEG3, CASC2) in Iranian breast cancer patients.
  • To explore the association between the expression of these genes and clinical variables of breast cancer.
  • To evaluate the diagnostic potential of these lncRNAs as biomarkers.

Main Methods:

  • Gene expression analysis of TP53, MEG3, CASC2, and PANDA in tumoral and non-tumoral breast tissue samples.
  • Statistical analysis to compare expression levels between groups and correlate with clinical parameters (Her2 status, tumor grade).
  • Receiver Operating Characteristic (ROC) curve analysis to assess diagnostic performance.

Main Results:

  • Significantly lower expression of TP53, MEG3, CASC2, and PANDA was observed in tumor tissues compared to non-tumoral tissues.
  • CASC2 expression was higher in Her2 1+ cases versus Her2-negative cases.
  • MEG3 and TP53 expression were lower in grade 2 tumors compared to grade 1.
  • CASC2 demonstrated the highest diagnostic performance (AUC=0.78, sensitivity=56.33%, specificity=88.73%).

Conclusions:

  • The study supports the involvement of TP53-related lncRNAs in breast cancer pathogenesis.
  • Downregulation of these lncRNAs may contribute to cancer development.
  • CASC2 shows promise as a potential diagnostic biomarker for breast cancer.
Keywords:
CASC2MEG3PANDATP53lncRNA

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