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Updated: Oct 6, 2025

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
Published on: September 12, 2025
Defining the interactomes of proteins involved in cytoskeletal dynamics using high-throughput proximity-dependent
Sarah Nahlé1,2, Laura Quirion1,2, Jonathan Boulais1
1Montreal Clinical Research Institute (IRCM), Montréal, QC H2W 1R7, Canada.
Abstract:
Proximity-dependent biotinylation (BioID) screens are excellent tools to capture in cellulo interactomes for a large variety of baits, including transient and weak affinity interactions, as well as localization-specific proximity components, which are much harder to detect with conventional approaches. Here, we describe the major starting steps and a detailed protocol on how to perform BioID in mammalian cells. We also describe the mass spectrometry procedure and the bioinformatics pipeline for the data analysis. For complete details on the use and execution of this profile, please refer to Bagci et al. (2020).
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