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Updated: Oct 6, 2025

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Published on: November 22, 2014
Protein inspired chemically orthogonal imines for linchpin directed precise and modular labeling of lysine in
Tularam Sahu1, Maheshwerreddy Chilamari1, Vishal Rai1
1Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhauri, Bhopal Bypass Road, Bhopal 462066, MP, India. vrai@iiserb.ac.in.
Abstract:
We report a chemoselective, site-selective, and modular technology for precision engineering of high-frequency lysine residues in native proteins. It enables a unique, unexplored reactivity landscape on the protein surface to facilitate their single-site modification. Further, the method presents bond-architecture flexibility and enables orthogonal tagging with probes of interest.
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