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Filtration

Filtration

Filtration is a physical separation process that involves passing a suspension through a porous medium to separate solids from fluids. During filtration, solids collect on the porous medium while liquids, also collectively known as the filtrate, pass through. The filtration medium is selected based on the filtration purpose, quantity, and nature of the precipitate. The general criteria for a suitable filtering medium are that it is inert, mechanically strong, nonabsorbent toward dissolved...

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Related Experiment Video

Updated: Oct 6, 2025

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Benchmarking a Microfluidic-Based Filtration for Isolating Biological Particles.

¹UNAM - National Nanotechnology Research Center, Bilkent University, 06800 Ankara, Turkey.

Langmuir : the ACS Journal of Surfaces and Colloids

|January 18, 2022

Summary

This summary is machine-generated.

This study presents a novel microfluidic device for isolating tiny exosome particles from complex biological fluids. The innovative design minimizes clogging and fouling, enabling efficient, multi-time use for bio-nanoparticle recovery.

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Last Updated: Oct 6, 2025

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

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Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

Area of Science:

Biomedical Engineering
Nanotechnology
Cell Biology

Background:

Isolating nanoparticles from complex biological fluids is vital for diagnostics and therapeutics.
Extracellular vesicles (EVs), particularly exosomes (30-100 nm), are crucial biomarkers but challenging to separate.
Existing membrane filtration methods suffer from clogging, low recovery, and fouling.

Purpose of the Study:

To design and assess a novel two-filter-integrated microfluidic device for efficient exosome isolation.
To overcome limitations of traditional membrane filtration, including clogging and fouling.
To enable multiple uses of the microfluidic platform for bio-nanoparticle recovery.

Main Methods:

A microfluidic device integrating dead-end and cross-flow filtration was designed.
Numerical simulations assessed pressure-drop and flow rate effects.
Exosomes were isolated from human embryonic kidney cells, mimicking complex biological samples.
Flow direction was altered to refresh membranes and minimize clogging.

Main Results:

The integrated microfluidic device effectively isolated exosome particles.
The design minimized recovery issues, clogging, and fouling.
Altering flow direction successfully refreshed membranes for repeated use.
The platform demonstrated robust performance for multiple bio-nanoparticle isolation cycles.

Conclusions:

The developed microfluidic device offers an efficient solution for exosome isolation from complex biological fluids.
This platform addresses key challenges in nanoparticle recovery, enabling reliable and reusable bio-separation.
The findings pave the way for improved diagnostic and therapeutic applications utilizing exosomes.