Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 9, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Thermally Modulated Metasurface Sensor for Dynamic and Time-Resolved Isolation of Extracellular Vesicles.

Beyza Nur Kucuk1,2, Eylul Gulsen Yilmaz1,2, Yusuf Aslan1,2

  • 1UNAM-National Nanotechnology Research Center, Bilkent University, Ankara, Turkey.

Advanced Materials (Deerfield Beach, Fla.)
|May 8, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perspectives in Nanotoxicity Studies for Carbon-Based Nanomaterials in 3D Platform.

Journal of biochemical and molecular toxicology·2026
Same author

Preoperative Systemic Inflammatory Marker Profile in Surgically Treated Intradural Spinal Tumors: A Retrospective Cohort Study.

Medicina (Kaunas, Lithuania)·2026
Same author

Therapeutic microneedles for myocardial repair.

Trends in biotechnology·2026
Same author

Multianalytical and Theoretical Tendency for the Clarification of the Binding of Lansoprazole and Albumin Protein.

ACS omega·2026
Same author

A wearable 3D-printed hollow microneedle device for pressure-driven interstitial fluid collection and testing.

Lab on a chip·2026
Same author

Spatiotemporal Confinements of Distance-Dependent Emitters for Enhancing Plasmonic Signals.

ACS applied materials & interfaces·2026
This summary is machine-generated.

This study introduces a novel sensor for isolating extracellular vesicles (EVs) using a thermoresponsive polymer. This method enhances EV purity and preserves their integrity for improved diagnostic applications.

Area of Science:

  • Biotechnology
  • Materials Science
  • Nanotechnology

Background:

  • Extracellular vesicles (EVs) are crucial for intercellular communication and hold significant diagnostic potential.
  • EV heterogeneity presents challenges in isolation and characterization, leading to low yield, contamination, and damage.
  • Current EV isolation methods often lack precision and can compromise vesicle integrity.

Purpose of the Study:

  • To develop a novel sensor for spatiotemporally controlled, label-free isolation of extracellular vesicles (EVs).
  • To enable gentle and efficient EV release for downstream analysis.
  • To advance EV-based biosensing and point-of-care diagnostics.

Main Methods:

  • Engineered a plasmonic metasurface sensor by repurposing nanograted optical disks.
Keywords:
PNIPAMextracellular vesicles (EVs)metasurfaceplasmonic sensorsmart polymer

More Related Videos

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing
12:01

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Published on: October 19, 2014

Related Experiment Videos

Last Updated: May 9, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing
12:01

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Published on: October 19, 2014

  • Functionalized the metasurface with poly(N-isopropylacrylamide) (PNIPAM) and anti-CD63 antibodies for selective EV capture.
  • Utilized a minute thermal change (near 35°C) for gentle EV release.

    • Main Results:

    • Achieved selective EV capture and gentle release with 87.03 ± 23.5% efficiency.
    • Demonstrated up to a 100-fold increase in EV purity compared to ultrafiltration.
    • Confirmed preserved vesicle morphology and marker expression via electron microscopy and Western blotting.

    Conclusions:

    • The thermoresponsive polymer-integrated plasmonic metasurface sensor offers a non-destructive, portable, and real-time solution for precise EV manipulation.
    • This platform significantly enhances EV purity and integrity, overcoming limitations of current isolation techniques.
    • The system holds promise for advancing EV-focused biosensing and liquid biopsy applications.