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Microbial cell surface engineering for high-level synthesis of bio-products
Xia Wu1, Jingyi Liu1, Zhiqiang Liu1
1School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, Shaanxi 710021, China.
Biotechnology Advances
|January 18, 2022
Summary
Engineering microbial cell surface layers offers a new strategy for enhancing bio-product synthesis. This approach optimizes microbial cell factories by targeting components like cell walls and membranes, improving yields without altering core metabolic pathways.
Area of Science:
- Microbiology
- Biotechnology
- Metabolic Engineering
Background:
- Microbial cell surface layers (cell membrane, cell wall, outer membrane, etc.) regulate essential material exchange.
- These layers significantly influence the production titers and yields of microbial bio-products.
- Emerging research targets cell surface components for metabolic engineering.
Purpose of the Study:
- To review recent advancements in engineering microbial cell surface components.
- To describe strategies for enhancing bio-product synthesis by targeting cell surface features.
- To discuss future directions in this field.
Main Methods:
- Summarizing recent research on microbial cell surface engineering.
- Describing engineering strategies in bacteria and yeasts.
- Analyzing impacts on mass transfer, protein expression, cell morphology, and resource allocation.
Main Results:
- Engineering cell surface components enhances mass transfer across the cell.
- Strategies improve protein expression and folding efficiency.
- Modulation of cell size and shape, and re-direction of cellular resources are achieved.
- These modifications lead to more efficient microbial cell factories.
Conclusions:
- Targeting microbial cell surface layers is a promising strategy for bio-product enhancement.
- This approach offers an alternative to direct metabolic pathway modification.
- Further research can optimize microbial cell factories for diverse bio-production applications.
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