Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neural Regulation01:37

Neural Regulation

40.5K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
40.5K
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

2.9K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: ATF7IP/SETDB1-mediated epigenetic programming regulates thymic homing and T lymphopoiesis of hematopoietic progenitors during embryogenesis.

Nature communications·2026
Same author

S100A4 characterize antigen-presenting cancer-associated fibroblasts and predicts surgical outcomes in relapsed ovarian cancer.

Therapeutic advances in medical oncology·2026
Same author

Single-Cell Analysis Links C7+ Cancer-Associated Fibroblasts with Incomplete Resection in Platinum-Sensitive Relapsed Ovarian Cancer.

Biomedicines·2025
Same author

A trispecific antibody engaging T cells with tumour and myeloid cells augments antitumour immunity.

Nature biomedical engineering·2025
Same author

Dynamic genome-wide mapping reveals how chromatin context shapes OGG1-mediated repair and related mutagenesis in human cells.

Nucleic acids research·2025
Same author

Stromal Cell-Mast Cell Communication Orchestrates Anti-Viral Immunity in the Meninges.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: Oct 6, 2025

Isolation of Adipose Tissue Immune Cells
07:09

Isolation of Adipose Tissue Immune Cells

Published on: May 22, 2013

36.5K

Eosinophils regulate intra-adipose axonal plasticity.

Xia Meng1,2,3, Xinmin Qian1,2,3, Xiaofan Ding1,2,3

  • 1Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China.

Proceedings of the National Academy of Sciences of the United States of America
|January 19, 2022
PubMed
Summary
This summary is machine-generated.

Eosinophils promote sympathetic nerve growth in white adipose tissue (WAT) by producing nerve growth factor (NGF). This interaction enhances energy expenditure during cold exposure, highlighting a link between immunity and metabolic regulation.

Keywords:
NGFaxonal plasticityeosinophilswhite adipose tissues

More Related Videos

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

3.8K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.4K

Related Experiment Videos

Last Updated: Oct 6, 2025

Isolation of Adipose Tissue Immune Cells
07:09

Isolation of Adipose Tissue Immune Cells

Published on: May 22, 2013

36.5K
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

3.8K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.4K

Area of Science:

  • Immunology
  • Neuroscience
  • Metabolism

Background:

  • Sympathetic innervation is crucial for regulating energy balance and metabolic adaptation.
  • The influence of the immune system on neuronal plasticity in adipose tissue remains largely unexplored.

Purpose of the Study:

  • To investigate the role of eosinophils in regulating nerve plasticity within white adipose tissues (WAT).
  • To elucidate the mechanisms by which immune cells influence sympathetic innervation and energy expenditure.

Main Methods:

  • Analysis of eosinophil abundance and nerve growth factor (NGF) production in WAT under cold conditions.
  • Assessment of cold-induced axonal outgrowth and adipose tissue beiging in eosinophil-deficient or NGF-deficient models.
  • Visualization of cellular interactions between sympathetic nerves, stromal cells, and eosinophils in human and mouse adipose tissues.
  • Investigation of signaling pathways involving adrenergic signals, IL-33, IL-5, and group 2 innate lymphoid cells (ILC2s).

Main Results:

  • Eosinophils increase in WAT during cold exposure and produce NGF, promoting axonal outgrowth and adipose tissue beiging.
  • Deletion of eosinophil-derived NGF or depletion of eosinophils impairs cold-induced sympathetic innervation and beiging.
  • A spatial proximity exists between sympathetic nerves, IL-33-expressing stromal cells, and eosinophils in adipose tissue.
  • Sympathetic signaling induces IL-33 release from stromal cells, which, via ILC2s and IL-5, leads to eosinophil recruitment.

Conclusions:

  • Eosinophils play a key role in enhancing sympathetic innervation of WAT through NGF production.
  • A feed-forward loop between sympathetic activity and type 2 immunity (involving IL-33 and eosinophils) promotes sympathetic innervation and energy expenditure.
  • This cross-talk between the nervous and immune systems offers novel insights into metabolic regulation and resilience to metabolic challenges.