Related Experiment Video

Updated: Oct 6, 2025

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.6K

JMJD2C-mediated long non-coding RNA MALAT1/microRNA-503-5p/SEPT2 axis worsens non-small cell lung cancer

Jun Zhang1, Mingliang Wang1, Jiashun Wang1

  • 1Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China.

Cell Death & Disease
|January 20, 2022
PubMed

Insights

Jumonji domain containing protein 2C (JMJD2C) epigenetically regulates non-small cell lung cancer (NSCLC) via the MALAT1/miR-503-5p/SEPT2 axis. Targeting this pathway offers a potential therapeutic strategy for NSCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Jumonji domain containing protein 2C (JMJD2C) is implicated in epigenetic regulation within cancer.
  • Non-small cell lung cancer (NSCLC) progression involves complex molecular mechanisms.
  • Understanding downstream pathways of JMJD2C is crucial for NSCLC research.

Purpose of the Study:

  • To elucidate the downstream mechanism of JMJD2C in NSCLC.
  • To investigate the role of the long non-coding RNA metastasis associated with lung adenocarcinoma transcript 1 (MALAT1)/microRNA-503-5p (miR-503-5p)/septin 2 (SEPT2) axis in NSCLC.
  • To assess the interactions between JMJD2C, MALAT1, miR-503-5p, and SEPT2 in NSCLC.

Main Methods:

  • Analysis of JMJD2C, MALAT1, miR-503-5p, and SEPT2 levels in NSCLC clinical tissues.
  • Loss-of-function and gain-of-function experiments in NSCLC cell lines (A549, H1299).
  • Assessment of molecular interactions using various assays.

Main Results:

  • Elevated JMJD2C, MALAT1, and SEPT2, with decreased miR-503-5p, observed in NSCLC.
  • JMJD2C depletion or MALAT1 knockdown, or miR-503-5p restoration, exhibited anti-tumor effects in vitro and in vivo.
  • JMJD2C directly binds to the MALAT1 promoter; MALAT1 interacts with miR-503-5p; miR-503-5p targets SEPT2.
  • Modulating MALAT1, SEPT2, or miR-503-5p counteracted JMJD2C-induced pro-tumor effects.

Conclusions:

  • The JMJD2C-mediated MALAT1/miR-503-5p/SEPT2 axis is a key player in NSCLC pathogenesis.
  • This axis contributes to NSCLC progression and severity.
  • Targeting the JMJD2C-MALAT1/miR-503-5p/SEPT2 pathway presents a potential therapeutic avenue for NSCLC.

More Related Videos

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

25.6K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.2K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.0K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.5K