Experimental study on the biocompatibility and osteogenesis induction ability of PLLA/DDM scaffolds

To investigate the characterization and function of a novel porous osteogenic material (PLLA / DDM) containing polylactic acid and demineralized dentin matrix. The surface morphology and composition of the material were observed by SEM and EDS. The physical characteristics of the material were detected by roughness and water contact angle analyses. The rate of weight loss and change in the pH value of the material were observed by scaffold degradation experiments. Four types of material were investigated: polylactic acid (PLLA) scaffold, demineralized dentin matrix (DDM) particles, PLLA/DDM scaffold and a blank control. The osteogenic effect and osteogenic characteristics of the new materials were explored through in vivo and in vitro osteogenic experiments. SEM analysis showed that DDM powder was uniformly distributed in the polylactic acid scaffold, and the water contact angle revealed that the water absorption of the porous scaffold was improved after the addition of DDM powder. The EDS results showed that the peak values of calcium and phosphorus were obviously increased after the addition of DDM powder, and the porosity test showed that the scaffold had higher porosity after the addition of DDM powder. Scaffold degradation experiments revealed that the scaffold gradually degraded with increasing time, and its pH value slightly increased. The results of cell culture and animal model experiments showed that the porous PLLA/DDM scaffold had good bio-compatibility and promoted cell proliferation and differentiation. In histological and micro-CT evaluations, the material showed good bio-compatibility, biodegradability and bone conductivity with host bone tissue in vivo. PLLA / DDM hybrid showed better performance than PLLA or DDM. The biocompatibility and cell growth promoting properties were stronger than those of single material.