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Published on: July 10, 2014
In vitro inhibition of demineralization from bioglass-containing adhesive and composite
Chan-Te Huang1,2, Nathan R Smith3, John O Burgess3
1Department of Dentistry, Chang Gung Memorial Hospital at Keelung, Keelung Ciity, Taiwan, resplendia@xuite.net.
Purpose:
To measure and compare the area of inhibition around a bioglass-containing adhesive and resin-based composite following an in vitro artificial caries model in human extracted teeth.
Methods:
Preparations were made at the CEJ of extracted human molars (40, n=10/material) and restored with combinations of a bioglass-containing adhesive (BA, Regen), a reference adhesive which served as a negative control (RA), a bioglass-containing composite (BC, Regen), and a reference composite which served as a negative control (RC): BABC, RABC, BARC, RARC. All materials were light-cured and then finished with a polishing disc. Teeth were incubated (37°C) for 24 hours in water. A demineralization solution composed of 0.1 M lactic acid, 3 mM Ca3(PO4)2, 0.1% thymol, and NaOH (to adjust pH= 4.5) and a remineralization solution composed of 1.5 mM Ca, 0.9 mM P, and 20 mM Tris(hydroxymethil)-aminomethane (pH= 7.0) were prepared. Specimens were placed in the demineralization solution for 4 hours followed by a remineralization solution for 20 hours and cycled daily for 30 days. The specimens were embedded, sectioned into 100 µm sections, and the interface between the adhesive/composite and root dentin margin was viewed with polarized light. A line was drawn parallel with the zone of demineralization for each tooth. The area of "inhibition" (defined as the area external to the line) or "wall lesion" (defined as the area internal to the line) was traced with internal image evaluation software and measured. Areas of inhibition were measured as positive values and areas of wall lesions were measured as negative values.
Results:
A one-way ANOVA found significant differences between materials for "inhibition/wall lesion" areas in root dentin (P< 0.001). Tukey post-hoc analysis ranked materials (µm2, mean± SD): BABC (3590± 2847)a RABC (1903± 1025)a, BARC (-792± 850)b, RARC (-2544± 1760)b.
Clinical Significance:
The use of bioglass-containing resin-based composite with or without a bioglass-containing adhesive demonstrated inhibition of demineralization at the restoration margin.
