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Serpinin in the Skin
Cristina Fraquelli1, Jasmine Hauzinger1, Christian Humpel2
1Department of Ophthalmology, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, Austria.
Biomedicines
|January 21, 2022
Summary
Serpinins, novel peptides from chromogranin A, are found in rat dorsal root ganglia and sensory nerves of the skin. These biologically active peptides may play a significant role in skin function.
Area of Science:
- Neuroscience
- Peptide Biology
Background:
- Serpinins are novel peptides derived from chromogranin A.
- They exist in three forms: free serpinin, serpinin-RRG, and pGlu-serpinin.
Purpose of the Study:
- To investigate the presence and origin of serpinins in rat skin.
- To determine the distribution and localization of serpinins in neural tissues and skin.
Main Methods:
- Western blot analysis to detect serinin-containing proteins.
- RP-HPLC and EIA to quantify serinin forms.
- Immunohistochemistry to assess cellular distribution and colocalization with substance P.
Main Results:
- Serinin-containing proteins were detected in rat trigeminal and dorsal root ganglia, but not in skin.
- All three serinin forms were found in dorsal root ganglia, with free serinin predominant in skin.
- Serinin-positive cells were abundant in dorsal root ganglia, colocalizing with substance P.
- Serinin immunoreactivity was observed in sensory and autonomic nerves in the skin, often colocalizing with substance P.
Conclusions:
- Serinin is present in the sensory innervation of the skin.
- Serinin peptides are constituents of both dorsal root ganglia and skin innervation.
- The biological activity of serpinins suggests functional significance in the skin.
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