Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

4.7K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

No usage-based linguistics without language use.

The Behavioral and brain sciences·2026
Same author

Inverse relationship between neoantigen clonality and T-cell activity reveals distinct immune phenotypes in HNSCC.

Journal of translational medicine·2026
Same author

An integrated single-cell and spatial proteotranscriptomics atlas of fibroblast-driven immunoregulation within the human adult oral cavity.

Cell press blue·2026
Same author

Exploring the Guessing-Game Experimental Paradigm: Inferences From Closed- Versus Open-Ended Semantic Space.

Cognitive science·2026
Same author

Loss of junctional plakoglobin (JUP) activates PI3K/AKT signaling in head and neck squamous cell carcinoma.

Medical oncology (Northwood, London, England)·2026
Same author

Three-dimensional assessment of condylar head position in CBCT scans before and after orthognathic surgery.

BMC oral health·2026

Related Experiment Video

Updated: Oct 6, 2025

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

11.7K

PKM2 Modulation in Head and Neck Squamous Cell Carcinoma.

Verena Boschert1, Jonas Teusch1, Urs D A Müller-Richter1,2,3

  • 1Department of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, D-97070 Würzburg, Germany.

International Journal of Molecular Sciences
|January 21, 2022
PubMed
Summary

Pyruvate kinase M2 (PKM2) is often phosphorylated in head and neck squamous cell carcinoma (HNSCC), influencing cancer metabolism and stemness. Targeting PKM2 activity shows varied effects on glycolysis, suggesting personalized treatment potential.

Keywords:
AMPKCD44Compound 3kDASA-58HNSCCPKM2TXNIPWarburg effectcancer metabolismglycolysishead and neck cancer

More Related Videos

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
12:03

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Published on: July 25, 2011

19.5K
Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
06:11

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

Published on: April 20, 2018

9.9K

Related Experiment Videos

Last Updated: Oct 6, 2025

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

11.7K
Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
12:03

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Published on: July 25, 2011

19.5K
Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
06:11

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

Published on: April 20, 2018

9.9K

Area of Science:

  • Biochemistry
  • Cancer Biology
  • Metabolic Regulation

Background:

  • Pyruvate kinase M2 (PKM2) is a key enzyme regulating the metabolic shift to aerobic glycolysis in cancer cells.
  • Head and neck squamous cell carcinoma (HNSCC) is characterized by high recurrence rates, necessitating a deeper understanding of its metabolic drivers.
  • PKM2 activity is modulated by allosteric regulators and posttranslational modifications, impacting cellular metabolism.

Purpose of the Study:

  • To investigate the role and modulation of PKM2 in HNSCC.
  • To examine PKM2 phosphorylation at Tyrosine 105 and its dependence on tyrosine kinase activity in HNSCC.
  • To assess the impact of PKM2 activators and inhibitors on HNSCC cell glycolysis and correlate PKM2 expression with cancer stemness markers like CD44.

Main Methods:

  • Real-time cell metabolic analysis to measure glycolysis.
  • Western blotting to detect PKM2 phosphorylation at Tyr105 and expression levels.
  • Treatment of HNSCC cells with PKM2 activator (DASA-58) and inhibitor (Compound 3k).
  • Analysis of epidermal growth factor (EGF) signaling and CD44 expression.

Main Results:

  • PKM2 is frequently phosphorylated at Tyrosine 105 in HNSCC cells, with levels dependent on tyrosine kinase activity and influenced by EGF.
  • PKM2 phosphorylation correlates with CD44 expression, suggesting a role in cancer stemness.
  • HNSCC cells exhibited increased glycolysis with PKM2 activation and decreased glycolysis with PKM2 inhibition, though responses varied among cell lines.
  • Individualized responses to PKM2 modulation were observed, indicating differential susceptibility to activation or inhibition.

Conclusions:

  • PKM2 phosphorylation at Tyr105 is a common event in HNSCC, linked to growth factor signaling and cancer stemness.
  • Modulation of PKM2 activity impacts HNSCC cell glycolysis, but responses are heterogeneous.
  • Further research into PKM2's role in HNSCC is crucial for developing targeted therapeutic strategies.