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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Oct 6, 2025

Flow Cytometry Purification of Mouse Meiotic Cells

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

Sorting single-cell microcarriers using commercial flow cytometers.

Joseph de Rutte¹, Robert Dimatteo², Sheldon Zhu³

¹Department of Bioengineering, University of California, Los Angeles, United States; Partillion Bioscience Corporation, Los Angeles, CA, United States.

SLAS Technology

|January 21, 2022

Summary

This summary is machine-generated.

Sub-nanoliter nanovials are compatible with fluorescence activated cell sorting (FACS), enabling scalable single-cell analysis. This breakthrough democratizes antibody and cell therapy discovery by leveraging existing flow cytometry equipment.

Keywords:

Flow cytometry Microfluidics Microparticles Nanovials Single-cell analysis

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A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Related Experiment Videos

Last Updated: Oct 6, 2025

Flow Cytometry Purification of Mouse Meiotic Cells

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Area of Science:

Biotechnology
Cell Biology
Analytical Chemistry

Background:

Biological discovery scale depends on assay vessels, from multi-well plates to microfluidics.
Existing methods for single-cell analysis have limitations in scale and detection sensitivity.

Purpose of the Study:

To assess the compatibility of sub-nanoliter "nanovials" with commercial fluorescence activated cell sorters (FACS).
To demonstrate the utility of nanovials for scalable single-cell assays using existing laboratory equipment.

Main Methods:

Utilized 3D structured microparticles as nanovials for cell isolation and single-cell assays.
Employed flow cytometry for fluorescence detection and measured dynamic ranges and detection limits.
Assessed sorting purity and throughput of nanovials on commercial FACS instruments.
Analyzed forward and side scatter signatures for gating cell-loaded nanovials.

Main Results:

Achieved detection limits of ~10,000 molecules per nanovial, outperforming fluorescence microscopy.
Demonstrated high sorting purity (93-99%) at throughputs up to 800 events/second.
Identified unique scatter signatures for effective gating of cell-containing nanovials.

Conclusions:

Nanovials are compatible with commercial FACS instruments, enabling robust single-cell analysis.
This technology democratizes single-cell assays for antibody and cell therapy discovery.
Leverages flow cytometry's analytical power for clone selection based on secreted products.