CellCelector™ as a platform in isolating primary B cells for antibody discovery

Area of Science:

• Immunology
• Biotechnology
• Antibody Engineering

Background:

• Traditional hybridoma technology for antibody discovery is labor-intensive and limits throughput.
• Directly mining the B-cell repertoire is crucial for efficient antibody discovery.
• Existing methods struggle to capture the full diversity of an immunized animal's antibody repertoire.

Purpose of the Study:

• To present a novel method for isolating individual antigen-specific primary B-cells.
• To demonstrate the utility of the CellCellector™ platform for antibody discovery.
• To identify and characterize novel antibodies against Epidermal Growth Factor Receptor (EGFR).

Main Methods:

• Utilized the CellCellector™ single-cell isolation platform.
• Isolated antigen-specific B-cells from XenoMouse® immunized with recombinant EGFR.
• Cloned and expressed heavy chain complementarity-determining region sequences into human IgG1 format.

Main Results:

• Successfully isolated 94 EGFR-specific B-cells from antibody-secreting cells.
• Identified 13 recombinant antibodies that bind to soluble and native forms of EGFR.
• Projected potential discovery of 1196 unique EGFR-specific antibodies from one immunized mouse.

Conclusions:

• The CellCellector™ platform enables direct interrogation of the B-cell pool for antibody discovery.
• This method significantly enhances the efficiency and throughput of identifying antigen-specific antibodies.
• The recovered antibodies demonstrate binding to native and recombinant EGFR, validating the platform's efficacy.