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Bacteria-engineered porous sponge for hemostasis and vascularization
Jie Bian1,2, Luhan Bao3, Xiaokang Gao4
1Department of Stomatology, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215006, Jiangsu, People's Republic of China.
Journal of Nanobiotechnology
|January 22, 2022
Summary
A novel oxidized bacterial nanocellulose sponge (OBNC-DFO) integrates hemostasis and vascularization for wound healing. This biomaterial accelerates clotting and promotes blood vessel formation, enhancing tissue regeneration and skin repair.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Hemostasis and vascularization are critical yet challenging to integrate in wound healing.
- Current approaches struggle to effectively address both early clot formation and subsequent tissue repair simultaneously.
Purpose of the Study:
- To develop an integrated hemostatic and vascularization-promoting biomaterial for enhanced wound repair.
- To create a novel hemostatic sponge using oxidized bacterial nanocellulose and desferrioxamine.
Main Methods:
- Constructed a hemostatic sponge (OBNC-DFO) via bacterial nanocellulose fermentation and TEMPO oxidation.
- Grafted desferrioxamine (DFO) to promote angiogenesis and enhance clot formation.
- Evaluated hemostatic and pro-coagulation effects in vitro and in vivo (rat models).
Main Results:
- OBNC-DFO demonstrated rapid water absorption (1.70 g/s) and promoted clot formation.
- Exhibited superior pro-coagulation effects compared to collagen sponges, reducing blood loss.
- Promoted endothelial cell proliferation and HIF-1α secretion, enhancing vascularization and skin regeneration.
Conclusions:
- The OBNC-DFO sponge effectively integrates hemostasis and vascularization.
- This biomaterial shows significant potential as a pro-regenerative repair agent for wounds.
- Achieved structural and functional skin regeneration in animal models.
