Successful startup of the single-stage PN-A (partial nitrification-anammox) process by controlling the oxygen supply

The single PN-A (partial nitrification-anammox) reactor offers a cost-effective solution for nitrogen removal. However, optimal control of the PN-A reactor is challenging due to the interactive mechanisms among the oxygen supply, bulk liquid DO (dissolved oxygen) concentration, and the balance of various functional bacterial species. In this study, a mathematical model was used to derive the optimal control variable for the maximum nitrogen removal, and an experimental PN-A reactor was operated to verify the model simulation results. The model simulation results indicate that the oxygen supply to the ammonium load ratio is the key factor to control the single-stage PN-A reactor for optimal TN removal. For optimal TN removal, the oxygen supply to the ammonium load ratio should be 1.9 mg O₂/mg N. The DO concentration is not the key control parameter to get the maximum TN removal as the optimal TN removal could be achieved under a wide range of DO concentration. The model simulation results were verified in the experimental PN-A reactor under oxygen transfer rate ([Formula: see text]) at 52 day^-1, HRT at 24 h, and ammonium load ratio of 0.55 kg N/(m³∙day).