Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

IR and UV–Vis Spectroscopy of Carboxylic Acids

4.7K
In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
4.7K
IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

6.2K
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the...
6.2K
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

7.5K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent...
7.5K
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

3.1K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
3.1K
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

2.6K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
2.6K
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

5.2K
Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range.
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intrinsic and Synergy of Carboxyl- and Amine-Bound Water at Electrified Interfaces and Its Contribution to Hydrogen Evolution.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Acoustic Rising Microbubbles for Efficient Liquid Operations.

Cyborg and bionic systems (Washington, D.C.)·2026
Same author

On-chip characterization of cell mechanics assisted by external physical fields and artificial intelligence.

Lab on a chip·2026
Same author

Lab on an end: Micromanipulation using the acoustohydrodynamic pillar array as an end effector.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Microhand Platform Equipped with Plate-Shaped End-Effectors Enables Precise Probing of Intracellular Structure Contribution to Whole-Cell Mechanical Properties.

Micromachines·2025
Same author

Advanced Microrobots Driven by Acoustic and Magnetic Fields for Biomedical Applications.

Cyborg and bionic systems (Washington, D.C.)·2025

Related Experiment Video

Updated: Oct 5, 2025

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

18.1K

Time-Resolved Infrared Spectroscopy with Multivariate Analysis on Photoinduced Proton Transfer in Aromatic

Masato Kondoh1, Hayato Honda1, Kei Togasaki1

  • 1Department of Chemistry, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571, Japan.

The Journal of Physical Chemistry. B
|January 24, 2022
PubMed
Summary

Photoinduced proton transfer in 1-Anthracen-2-yl-3-phenylurea (2PUA) acetate complexes was studied. The reaction mechanism remained consistent across varying concentrations, revealing key insights into aromatic urea photochemistry.

More Related Videos

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.3K
Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
10:02

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Published on: February 18, 2014

9.1K

Related Experiment Videos

Last Updated: Oct 5, 2025

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

18.1K
Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.3K
Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
10:02

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Published on: February 18, 2014

9.1K

Area of Science:

  • Photochemistry
  • Supramolecular Chemistry
  • Spectroscopy

Background:

  • 1-Anthracen-2-yl-3-phenylurea (2PUA) forms hydrogen-bonded complexes with acetate anions (AcO-).
  • Understanding photoinduced reactions in such complexes is crucial for developing new functional materials.

Purpose of the Study:

  • To investigate the photoinduced reaction mechanism of the 2PUA-AcO- complex in DMSO using time-resolved infrared spectroscopy.
  • To elucidate the role of concentration on the photoinduced proton transfer dynamics.

Main Methods:

  • Nanosecond time-resolved infrared (TR-IR) spectroscopy was employed to study the 2PUA-AcO- complex in DMSO.
  • Singular value decomposition (SVD) analysis was used to interpret concentration-dependent TR-IR spectra.

Main Results:

  • TR-IR spectra were explained by a photoinduced proton transfer model, consistent across different concentration conditions.
  • An unexpected signal related to free acetate concentration was observed under excess acetate conditions.
  • SVD analysis confirmed the proton transfer mechanism is independent of concentration.

Conclusions:

  • The photoinduced proton transfer in the 2PUA-AcO- complex occurs consistently, irrespective of concentration variations.
  • Both the 2PUA-AcO- complex and its tautomer species exhibit significant interactions with DMSO solvent molecules in their ground states.