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Discovering the Role of FZD4 Gene in Human Cutaneous Squamous Cell Carcinoma

Ke Zhang1, Qun Lv2, Liming Li2

  • 1Department of Dermatologic Surgery, Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing, Jiangsu, China.

Indian Journal of Dermatology
|January 24, 2022
PubMed
Abstract

Insights

Frizzled 4 (FZD4) is downregulated in cutaneous squamous cell carcinoma (CSCC). Overexpressing FZD4 inhibits CSCC cell proliferation and promotes apoptosis, suggesting FZD4 acts as a tumor suppressor.

Area of Science:

  • Oncology
  • Molecular Biology
  • Dermatology

Background:

  • Frizzled 4 (FZD4) is a Wnt protein receptor crucial for downstream signaling pathways.
  • FZD4-Wnt interactions are implicated in various cancers.
  • The role of FZD4 in cutaneous squamous cell carcinoma (CSCC) remains understudied.

Purpose of the Study:

  • To investigate the relationship between FZD4 expression and tumor cell proliferation in CSCC.
  • To examine the association between FZD4 levels and apoptosis rates in CSCC.

Main Methods:

  • Quantified FZD4 mRNA expression in CSCC tissues and controls.
  • Assessed Colo16 cell proliferation using CCK-8 assay.
  • Determined Colo16 cell viability via flow cytometry after siRNA and plasmid transfection.

Main Results:

  • FZD4 expression was significantly downregulated in CSCC tissues and cell lines compared to controls.
  • Overexpression of FZD4 inhibited proliferation of Colo16 cells.
  • FZD4 overexpression promoted apoptosis in Colo16 cells.

Conclusions:

  • FZD4 may function as a tumor suppressor gene in the development of CSCC.
  • FZD4 downregulation could contribute to CSCC pathogenesis.
Keywords:
CarcinogenesisFZD4Wnt signaling pathwaycutaneous squamous cell carcinomatumor suppressor gene

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