Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Apoptosis01:30

Apoptosis

12.1K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
12.1K
Overview of Cell Death01:30

Overview of Cell Death

7.9K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.9K
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

6.7K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.7K
Necrosis01:16

Necrosis

5.0K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
5.0K
Autophagic Cell Death01:18

Autophagic Cell Death

3.6K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.6K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

7.0K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo membrane engineering traps Gd-based MRI contrast agents for detecting microhepatocellular carcinoma.

Science advances·2026
Same author

GTPase Rac Regulates Conidiation, AFB1 Production and Stress Response in Pathogenic Fungus <i>Aspergillus flavus</i>.

Toxins·2022
Same author

The Trends of Psychological Status of People Entering from High-Risk Areas of COVID-19 Coronavirus During the Quarantine in Dedicated Hotels: A Longitudinal Survey Study from Guangzhou, China.

Risk management and healthcare policy·2021
Same author

Predictors of Seronegative Conversion After Centralized Management of Syphilis Patients in Shenzhen, China.

Frontiers in public health·2021
Same author

Development and utility of a close contact information management system for the COVID-19 pandemic.

BMC public health·2021
Same author

A Tubular Belt and a Möbius Strip with Dynamic Joints: Synthesis, Structure, and Host-Guest Chemistry.

Organic letters·2021
Same journal

In silico Evaluation of Marine Phage Tail Proteins as Inhibitors of β-Lactamases: A Novel Approach to Combat Antibiotic Resistance.

Indian journal of microbiology·2026
Same journal

Promoted Efficacy for Breast Cancer Cell Line Killing via the Combination of Metformin and GM-CSF-Expressing Oncolytic Virus.

Indian journal of microbiology·2026
Same journal

Bacterial L-forms: Key Mechanisms of Drug Resistance, Disease Recurrence, and Immune Evasion.

Indian journal of microbiology·2026
Same journal

Challenges with Bioaugmentation and Field-Scale Application of Bioremediation Processes for Petroleum-Contaminated Sites: A Review.

Indian journal of microbiology·2026
Same journal

Deciphering Distinct Pathogenetic Strategies Employed by <i>Shigella</i> Serotypes Using Human Intestinal Organoids.

Indian journal of microbiology·2026
Same journal

Biological Approaches to Carbon Sequestration and Bioenergy Production.

Indian journal of microbiology·2026
See all related articles

Related Experiment Video

Updated: Oct 5, 2025

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

8.8K

Cellular Apoptosis Induced by Deoxynivalenol.

Qing'ai Chen1, Ying Cui2, Jiaru Zhao2

  • 1College of Tourism and Leisure Management, Fujian Business University, Fuzhou, 350012 Fujian China.

Indian Journal of Microbiology
|January 24, 2022
PubMed
Summary
This summary is machine-generated.

Deoxynivalenol (DON) causes cell damage and apoptosis in Hela and Chang liver cells, impacting cellular health. This mycotoxin also showed toxicity in mice, affecting organs and increasing liver enzymes.

Keywords:
In vivoApoptosisDeoxynivalenolIn vitro

More Related Videos

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
12:55

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Published on: February 16, 2015

18.2K
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
13:21

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

9.2K

Related Experiment Videos

Last Updated: Oct 5, 2025

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

8.8K
Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
12:55

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Published on: February 16, 2015

18.2K
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
13:21

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

9.2K

Area of Science:

  • Toxicology
  • Cell Biology
  • Food Safety

Background:

  • Deoxynivalenol (DON) is a mycotoxin produced by Fusarium species, posing risks through contaminated food crops and animal products.
  • Understanding DON's cellular effects is crucial for assessing human health risks.

Purpose of the Study:

  • To investigate the cellular apoptosis induced by deoxynivalenol (DON) in Hela and Chang liver cells.
  • To evaluate the in vitro and in vivo toxicity of DON.

Main Methods:

  • Cell treatment with varying DON concentrations and incubation times.
  • Analysis using MTT assay, fluorescence microscopy, flow cytometry, and Western blot.
  • In vivo toxicity assessment in mice.

Main Results:

  • DON induced significant morphological changes in Hela and Chang liver cells.
  • Apoptosis was promoted by DON in a dose- and time-dependent manner, involving Caspase-3 activation.
  • DON exposure led to decreased Glutathione (GSH), increased Malondialdehyde (MDA) indicating oxidative stress, and toxicity in mice.

Conclusions:

  • Deoxynivalenol is toxic to tested cell lines and mice.
  • DON induces apoptosis via a Caspase-3 related pathway.
  • DON exposure causes oxidative stress and organ damage.