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Updated: Oct 5, 2025

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research
Published on: August 9, 2022
Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM
1Center for Plant Aging Research, Institute for Basic Science, Daegu 42988, Republic of Korea.
Abstract:
Ribosomes are composed of a mix of ribosomal RNAs and proteins; this composition varies depending on time, condition, and organism. Here, we present an optimized protocol for preparation of intact ribosomes from the skeletal muscle of the turquoise killifish. We also detail the steps for ribosome quantification and cryo-EM grid preparation. This protocol can enable the identification of heterogeneous ribosome structures that vary by fish age or in response to specific conditions.

