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Monitoring influenza virus oseltamivir resistance - our experience to date

L Nováková, M Havlíčková

    Epidemiologie, Mikrobiologie, Imunologie : Casopis Spolecnosti Pro Epidemiologii a Mikrobiologii Ceske Lekarske Spolecnosti J.E. Purkyne
    |January 25, 2022
    PubMed
    Abstract

    Insights

    Routine surveillance detected oseltamivir resistance in 9 out of 326 influenza virus isolates. Monitoring antiviral resistance is crucial for controlling drug use and tracking spread in the population.

    Area of Science:

    • Virology
    • Infectious Diseases
    • Public Health

    Background:

    • Influenza virus resistance monitoring is a routine surveillance activity.
    • The National Reference Laboratory for Influenza and Non-Influenza Respiratory Viral Diseases (NRL/INI) conducts this monitoring.
    • The aim is to detect neuraminidase inhibitor (oseltamivir) resistance in influenza patients.

    Purpose of the Study:

    • To detect oseltamivir resistance in influenza virus isolates.
    • To assess the prevalence of antiviral resistance in circulating influenza strains.

    Main Methods:

    • 326 influenza virus isolates from 2013/2014 to 2019/2020 seasons were analyzed.
    • Virus isolation was performed using Madin-Darby canine kidney (MDCK) cells.
    • Oseltamivir resistance was tested using the NA-Star Influenza Neuraminidase Inhibitor Resistance Detection Kit.

    Main Results:

    • Nine out of 326 (approximately 2.8%) influenza virus isolates were found to be oseltamivir resistant.
    • Resistant strains exhibited average IC50 values 100 times higher than sensitive strains.

    Conclusions:

    • Influenza virus resistance monitoring is vital for guiding antiviral prescription and surveillance.
    • Monitoring is essential in both hospitalized and outpatient settings to detect emerging resistance.
    • Early detection of resistant strains aids in controlling antiviral drug resistance spread within the population.
    Keywords:
    Mutationinfluenza A and Bmutationoseltamivirresistancesurveillance

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