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Transience of interference in an immunoassay measuring serum levels of beta-D-glucan
Harry E Prince1, Hollis J Batterman1, Susan E Realegeno1
1Quest Diagnostics, San Juan Capistrano, CA, USA.
Abstract:
Some sera tested for 1-3-beta-D-glucan to identify invasive fungal infections exhibit interference. To assess interference transience, we evaluated results for 426 patients with an interference sample followed by a later sample. Interference was transient for 73% of patients (later sample negative or positive); median time between samples was 8 days.
Insights
Interference in 1-3-beta-D-glucan testing for invasive fungal infections is often transient. For most patients, a follow-up sample confirmed the initial interference was temporary, with a median resolution time of 8 days.
Area of Science:
- Clinical chemistry
- Mycology
- Infectious diseases
Background:
- 1-3-beta-D-glucan is a biomarker for invasive fungal infections.
- Assays for this biomarker can be subject to interference, potentially impacting diagnostic accuracy.
Purpose of the Study:
- To evaluate the transient nature of interference in 1-3-beta-D-glucan testing.
- To determine the time course of interference resolution in patient samples.
Main Methods:
- Analysis of results from 426 patients with an initial sample showing interference.
- Comparison of initial interference samples with subsequent patient samples.
- Assessment of interference status (positive/negative) in later samples.
Main Results:
- Interference was observed in initial samples from 426 patients.
- Follow-up samples indicated transient interference in 73% of these patients.
- The median time between the initial interference sample and a resolved sample was 8 days.
Conclusions:
- Interference in 1-3-beta-D-glucan testing is frequently transient.
- This transient nature suggests that re-testing or careful interpretation of initial results may be warranted.
- Understanding interference patterns aids in reliable diagnosis of invasive fungal infections.
