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Related Concept Videos

The Cell Cycle Control System01:28

The Cell Cycle Control System

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The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
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Positive Regulator Molecules01:45

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To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
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Molecular Factors Affecting Cell Division01:27

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Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
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Cells Coordinate Growth and Proliferation02:36

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Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
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Interphase00:54

Interphase

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The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the cell cycle (G1, S, and G2), and mitosis (M). During interphase, which takes up about 95 percent of the duration of the eukaryotic cell cycle, cells grow and replicate their DNA in preparation for mitosis.
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M-Cdk Drives Transition Into Mitosis02:15

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Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
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M cyclin...
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

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Advances and enabling technologies for phase-specific cell cycle synchronisation.

Pritam Bordhan1,2, Sajad Razavi Bazaz1,2, Dayong Jin2

  • 1School of Biomedical Engineering, University of Technology Sydney, Sydney, New South Wales 2007, Australia. majid.warkiani@uts.edu.au.

Lab on a Chip
|January 25, 2022
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Summary
This summary is machine-generated.

Cell cycle synchronisation isolates cells to specific phases for research. This review covers methods from conventional to microfluidics, aiming for high-throughput, viable cell synchrony.

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Area of Science:

  • Cell biology
  • Biotechnology
  • Biophysics

Background:

  • Cell cycle synchronisation is crucial for isolating specific cell cycle phases from asynchronous cultures.
  • Applications include targeted gene editing, drug efficacy studies, and understanding cell cycle regulation.
  • Ideal methods require broad applicability, sustained synchrony, cell viability, and robustness.

Purpose of the Study:

  • To review and categorize cell cycle synchronisation approaches.
  • To discuss operational principles and performance efficiencies of various techniques.
  • To highlight technological trends and future perspectives in cell synchronisation.

Main Methods:

  • Categorization of cell cycle synchronisation techniques.
  • Discussion of conventional methods and recent microfluidics-based systems.
  • Analysis of advances and technological development trends.

Main Results:

  • Conventional and microfluidics-based cell synchronisation systems were evaluated.
  • Performance efficiencies and operational principles were discussed.
  • Advances in high-throughput synchronisation and future hybrid modalities were highlighted.

Conclusions:

  • Cell synchronisation techniques have evolved significantly, with microfluidics offering new possibilities.
  • Challenges remain in achieving high-throughput, phase-specific synchrony with minimal cellular impact.
  • Future platforms may integrate hybrid modalities for enhanced cell synchronisation efficiency and versatility.