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Cell-derived Extracellular Matrix Proteins in Colloidal Microgel as a Self-Assembly Hydrogel for Regenerative
Hacer Aksel1, Debanjan Sarkar2, Meng Hsuan Lin2
1Departments of Periodontics and Endodontics, School of Dental Medicine, University at Buffalo, New York.
Journal of Endodontics
|January 25, 2022
Summary
Colloidal microgels support cell differentiation and organization. Cell-derived extracellular matrix (ECM) in microgels promotes odontogenic differentiation, while commercial ECM promotes angiogenic properties in a 3D culture model.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Investigated colloidal microgels for cell differentiation in a 3D culture model.
- Utilized cell-derived extracellular matrix (ECM) proteins and commercial hydrogels.
Purpose of the Study:
- To evaluate colloidal microgels for angiogenic and odontogenic differentiation.
- To assess the influence of ECM proteins on cell behavior within hydrogels.
Main Methods:
- Determined viscoelastic properties of human dental pulp.
- Cultured dental pulp stem cells (DPSCs) in colloidal microgels with ECM proteins or GelMA.
- Assessed cell viability, odontogenic differentiation (gene expression, mineralization, ALP, calcium), and endothelial cell angiogenesis (tubule formation).
Main Results:
- Dental pulp exhibited solid-like viscoelastic properties.
- DPSC-derived ECM showed higher collagen and GAG content than MaxGel.
- Colloidal microgels supported endothelial cell tubule formation, unlike GelMA.
- DPSC-derived ECM in microgels upregulated odontogenic genes, while MaxGel upregulated angiogenic genes.
Conclusions:
- Colloidal microgels facilitate cellular organization, potentially improving nutrient supply in root canals.
- The bioactivity of cell-derived ECM can be modulated by external stimuli.
