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MicroRNA detection using light-up aptamer amplification based on nuclease protection transcription

Yoonseo Hong1,2, Do-Eun Kim1,2, Yu-Jin Park2

  • 1Bionanotechnology Research Center, Korea research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea. bjy8349@kribb.re.kr.

Chemical Communications (Cambridge, England)
|January 26, 2022
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MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...