Related Experiment Videos
Determination of adriamycin and its fluorescent metabolites in human plasma by HPLC
R Mahdadi1, M Lhermitte, J J Lafitte
1Laboratoire de Toxicologie, Faculté de Pharmacie de Lille, France.
Biomedical Chromatography : BMC
|February 1, 1987
Abstract:
We describe a method for measuring adriamycin and its metabolites, adriamycinol and adriamycinone in plasma, using reversed phase HPLC and fluorescence detection. The lower limit of detection is approximately 1 ng/mL for each compound. An extraction technique for serum is described which is capable of an almost equal recovery (greater than 93%) of adriamycin and metabolites without interference from endogenous components of plasma and from other common drugs. Within-day and day to day coefficients of variation are estimated.