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Microarray expression profile and functional analysis of circular RNAs in choroidal neovascularization
Jiancheng Huang1,2, Meng Chen2,3, Kai Xu2,3
1Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University, State Key Laboratory of Reproductive Medicine, Nanjing, Jiangsu 210029, China.
Journal of Biomedical Research
|January 27, 2022
Summary
Circular RNAs (circRNAs) are newly identified in choroidal neovascularization (CNV), a major cause of vision loss in age-related macular degeneration (AMD). These dysregulated circRNAs, including circ_15752, may play a role in CNV development and could serve as diagnostic biomarkers.
Area of Science:
- Ophthalmology
- Molecular Biology
- Genomics
Background:
- Choroidal neovascularization (CNV) is a primary cause of vision impairment in age-related macular degeneration (AMD).
- The precise molecular mechanisms driving CNV progression remain largely unknown.
- Circular RNAs (circRNAs) represent a novel class of non-coding RNAs with significant biological functions.
Purpose of the Study:
- To identify circRNAs associated with CNV.
- To investigate the functional roles and regulatory networks of differentially expressed circRNAs in CNV.
- To explore the potential of circRNAs as biomarkers for CNV.
Main Methods:
- Microarray analysis was employed to detect differentially expressed circRNAs in a murine CNV model.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed.
- Bioinformatic tools (TargetScan, miRanda) were used to predict miRNA targets and construct regulatory networks.
Main Results:
- 117 circRNAs were found to be differentially expressed in the CNV model.
- Host genes of these circRNAs were linked to neurogenesis, extracellular matrix (ECM), and binding functions.
- circ_15752 was identified as a potential promoter of vascular endothelial cell proliferation, migration, and tube formation, crucial for angiogenesis.
Conclusions:
- Dysregulated circRNAs are implicated in the pathogenesis of CNV.
- circRNAs, particularly circ_15752, may be critical regulators of angiogenesis in CNV.
- circRNAs show promise as potential diagnostic biomarkers for CNV.
