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[Genetic Engineering Systems to Study Human Viral Pathogens from the Coronaviridae Family]

S O Galkin1,2, A N Anisenko1,2,3, O A Shadrina2,3

  • 1Bioengineering and Bioinformatics Department, Moscow State University, Moscow, 119991 Russia.

Molekuliarnaia Biologiia
|January 27, 2022
PubMed

Insights

This review summarizes genetic engineering systems for studying SARS-CoV-2 and other coronaviruses. It covers viral genome manipulation and inhibitor screening for developing effective COVID-19 treatments.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetic Engineering

Background:

  • The COVID-19 pandemic, caused by SARS-CoV-2, highlighted the need for advanced research tools.
  • Understanding coronavirus structure and life cycles is crucial for therapeutic development.
  • Existing genetic engineering systems require evaluation for studying SARS-CoV-2 and related viruses.

Purpose of the Study:

  • To review current genetic engineering systems for studying SARS-CoV-2 and other Coronaviridae.
  • To provide an overview of coronavirus structure and life cycle relevant to genetic manipulation.
  • To facilitate high-throughput screening of potential viral inhibitors.

Main Methods:

  • Literature review of genetic engineering systems for coronaviruses.
  • Compilation of data on SARS-CoV-2 and other Coronaviridae studies.
  • Synthesis of information on viral structure and replication.

Main Results:

  • Summary of various genetic engineering approaches applicable to coronaviruses.
  • Overview of key features of coronavirus genome manipulation.
  • Identification of systems suitable for inhibitor screening.

Conclusions:

  • Effective genetic engineering systems are vital for advancing coronavirus research.
  • Further development of these systems can accelerate the discovery of antiviral therapies.
  • This review provides a foundation for selecting appropriate tools for coronavirus studies.
Keywords:
COVID-19SARS-CoV-2pseudovirusesreplicons

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