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Wounding Zebrafish Larval Epidermis by Laceration
Andrew S Kennard1,2, Christopher K Prinz2,3, Ellen C Labuz1,2
1Biophysics Program, Stanford University, Stanford, CA, USA.
Bio-Protocol
|January 28, 2022
Summary
We developed a new "tissue laceration" method in zebrafish to study rapid cell migration to wounds. This technique effectively prompts a strong cellular response for observing early wound healing dynamics.
Area of Science:
- Regenerative Medicine
- Developmental Biology
- Cell Biology
Background:
- Wound healing involves cell migration to repair tissue damage.
- The rapid, early cellular response to injury in vivo remains understudied.
- Zebrafish larvae offer high spatiotemporal resolution for live imaging of these processes.
Purpose of the Study:
- To develop a novel wounding method for larval zebrafish.
- To investigate the rapid cellular response to epidermal injury.
- To enable real-time observation of cell migration dynamics.
Main Methods:
- Developed a "tissue laceration" technique using a glass needle to wound zebrafish tailfins.
- Generated full-thickness epidermal wounds.
- Compatible with live imaging and downstream analyses.
Main Results:
- Tissue laceration elicits a rapid migratory wound response within seconds.
- Cells migrate from distances up to several hundred micrometers.
- This method induces a more robust early response than tail transection.
Conclusions:
- Tissue laceration is an effective and accessible method for studying rapid wound healing.
- It allows interrogation of how cells detect and respond to distant injury.
- Facilitates research into the mechanisms of early cell migration in wound repair.
