An ultrasensitive colloidal gold immunosensor to simultaneously detect 12 beta (2)-adrenergic agonists

Area of Science:

• Veterinary science
• Analytical chemistry
• Immunology

Background:

• Beta (2)-adrenergic agonists are used to promote growth in livestock.
• Detection of these compounds is crucial for food safety and regulatory compliance.
• Existing detection methods can be time-consuming and require specialized equipment.

Purpose of the Study:

• To develop a selective monoclonal antibody against 12 beta (2)-adrenergic agonists.
• To create a rapid, on-site detection method for these agonists in animal samples.
• To assess the sensitivity and applicability of the developed assay.

Main Methods:

• Preparation of a selective monoclonal antibody against 12 beta (2)-adrenergic agonists.
• Design and synthesis of three haptens, including a novel hapten (hapten S3) with a carboxyl group spacer.
• Development of a monoclonal antibody-based colloidal gold immunochromatographic test strip.

Main Results:

• The developed monoclonal antibody showed high selectivity for 12 beta (2)-adrenergic agonists.
• The half-maximal inhibitory concentration (IC50) values ranged from 0.35 ng/mL (Salbutamol) to 4.89 ng/mL (Clenproperol).
• The immunochromatographic test strip demonstrated suitability for rapid screening of these agonists in swine urine and lamb samples.

Conclusions:

• A novel monoclonal antibody and an immunochromatographic assay were successfully developed.
• The assay provides a sensitive and specific tool for on-site detection of beta (2)-adrenergic agonists.
• This method is valuable for rapid screening in food safety and animal health monitoring.