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ACE2-based capacitance sensor for rapid native SARS-CoV-2 detection in biological fluids and its correlation with
A Georgas1, E Lampas2, D P Houhoula3
1National Technical University of Athens, Zografou Campus 9, Iroon Polytechniou str Zografou, 15780, Greece.
Biosensors & Bioelectronics
|January 29, 2022
Summary
A new antibody-free biosensor detects SARS-CoV-2 by measuring electrical capacitance. This rapid, simple test accurately identifies the virus in patient samples, offering a promising tool for point-of-care diagnostics.
Area of Science:
- Biomedical Engineering
- Infectious Disease Diagnostics
- Nanotechnology
Background:
- The global threat of SARS-CoV-2 necessitates rapid and effective diagnostic tools.
- Current diagnostic methods require timely development of advanced technologies.
- Effective monitoring of viral spread is crucial for public health.
Purpose of the Study:
- To develop and test an antibody-free biosensor for SARS-CoV-2 detection.
- To immobilize ACE2 protein on a gold interdigitated electrode for virus targeting.
- To evaluate the biosensor's performance in laboratory and clinical settings.
Main Methods:
- Development of an antibody-free biosensor utilizing ACE2 protein immobilization.
- Detection of SARS-CoV-2 structural spike protein using electrical effective capacitance measurements.
- Correlation of biosensor results with real-time PCR for validation.
Main Results:
- The biosensor demonstrated a limit of detection of 750 pg/μL/mm² for the spike protein.
- Accurate discrimination between positive (7 samples) and negative (16 samples) patient samples (swab and saliva).
- Capability for semi-quantitative measurement with three distinct detection areas (negative, weakly positive, positive).
Conclusions:
- The developed biosensor offers a rapid (<2 min) and simple point-of-care screening test for SARS-CoV-2.
- The antibody-free design and electrical detection method present a novel approach to viral diagnostics.
- The biosensor shows potential for widespread use in managing the SARS-CoV-2 pandemic.
