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Updated: Oct 5, 2025

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
Published on: November 1, 2024
Synergy and sensitivity-balance in concatenating experiments in NO relaxation delay NMR (NORD)
István Timári1, Tamás Milán Nagy2,3, Katalin E Kövér2,3
1Department of Organic Chemistry, University of Debrecen, Egyetem tér 1, Debrecen H-4032, Hungary.
Abstract:
The NMR experiment design strategy of NO Relaxation Delay (NORD), introduced mostly as an idealized theoretical approach, is extended and put to practical use by considering synergy and sensitivity-balance in concatenation of experiments. It is illustrated by a novel experiment, NORD {HMBC}-{HSQC}-{TOCSY}, where magnetization of non-13C attached protons effectively is channeled from the TOCSY spectrum toward primarily the least sensitive spectrum of HMBC. The experiment is expected to find its place as a full-package NMR method for metabolomics, carbohydrates, peptides and small-molecules in general.
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