Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generic Protocols for the Analytical Validation of Next-Generation Sequencing-Based Circulating Tumor DNA Assays, Version 2.0.

JCO precision oncology·2026
Same author

Generic Protocols for Analytical Validation of Tumor-Informed Circulating Tumor DNA Assays for Molecular Residual Disease: The Blood Profiling Atlas in Cancer's Molecular Residual Disease Analytical Validation Working Group Consensus Recommendation.

JCO precision oncology·2026
Same author

Lexicon for Clonal Hematopoiesis in Liquid Biopsy.

Clinical and translational science·2025
Same author

Sinking Stones and Fluorescent Undertones: Intraoperative Parathyroid Identification Technique.

Annals of thoracic surgery short reports·2025
Same author

EET-Based Therapeutics Mitigate Sorafenib-Associated Glomerular Cell Damage.

Biomolecules·2025
Same author

Augmenting precision medicine via targeted RNA-Seq detection of expressed mutations.

NPJ precision oncology·2025
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Oct 5, 2025

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors
05:58

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Published on: August 16, 2024

3.2K

Microdissection Methods Utilizing Single-Cell Subtype Analysis and the Impact on Precision Medicine.

Donald J Johann1, Sarah Laun2, Ik Jae Shin1

  • 1Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 30, 2022
PubMed
Summary
This summary is machine-generated.

Maximizing tumor tissue from biopsies is crucial for precision oncology. This new method uses optimized microdissection and droplet digital PCR for efficient single-cell subtype analysis in solid tumors.

Keywords:
BiomarkerCancerDroplet digital PCR (ddPCR)Laser capture microdissection (LCM)Lung cancerPrecision medicinePrecision oncologySingle-cell analysisTumor

More Related Videos

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K
Author Spotlight: Shear Assay Protocol for the Determination of Single-Cell Material Properties
08:19

Author Spotlight: Shear Assay Protocol for the Determination of Single-Cell Material Properties

Published on: May 19, 2023

1.9K

Related Experiment Videos

Last Updated: Oct 5, 2025

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors
05:58

Author Spotlight: Exploring Strategies for Successful Immune Response Against Tumors

Published on: August 16, 2024

3.2K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K
Author Spotlight: Shear Assay Protocol for the Determination of Single-Cell Material Properties
08:19

Author Spotlight: Shear Assay Protocol for the Determination of Single-Cell Material Properties

Published on: May 19, 2023

1.9K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Precision oncology relies on accurate tumor genotyping for targeted therapies.
  • National Comprehensive Cancer Network guidelines recommend molecular testing for specific cancers.
  • Current methods often face limitations with small diagnostic biopsy samples.

Purpose of the Study:

  • To develop an improved method for solid tumor genotyping from limited biopsy material.
  • To enhance the utilization of tumor tissue for molecular analysis in precision medicine.
  • To demonstrate a cost- and time-efficient genotyping approach.

Main Methods:

  • Optimization of the microdissection process for formalin-fixed paraffin-embedded (FFPE) specimens.
  • Single-cell subtype analysis based on shared characteristics like immunostaining or spatial location.
  • Synergistic application of microdissection with droplet digital PCR (ddPCR) for molecular analysis.

Main Results:

  • Demonstrated a methodology for genotyping solid tumors from small biopsy samples.
  • Achieved time- and cost-efficient molecular analysis.
  • Successfully utilized immunostaining as an example for target identification.

Conclusions:

  • The described methodology improves tumor tissue utilization for genotyping.
  • This approach supports precision oncology by enabling detailed molecular analysis from limited samples.
  • Optimized microdissection combined with ddPCR offers an efficient solution for advanced cancer diagnostics.