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Related Experiment Video

Updated: Oct 5, 2025

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

Node-Pore Sensing for Characterizing Cells and Extracellular Vesicles.

Thomas Carey¹, Brian Li¹, Lydia L Sohn^2,3

¹The UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, Berkeley, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 30, 2022

Summary

This summary is machine-generated.

Node-Pore Sensing (NPS) offers versatile cell analysis and extracellular vesicle (EV) detection. This powerful technique precisely measures cell size and mechanical properties using microfluidic channels and modulated current pulses.

Keywords:

Cancer cells Extracellular vesicles Mechano-phenotyping Node-pore sensing Resistive-pulse sensing Tumor marker

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Last Updated: Oct 5, 2025

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

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Published on: December 2, 2022

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

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Published on: October 19, 2014

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Node-Pore Sensing (NPS) is a versatile technique for analyzing cells and detecting extracellular vesicles (EVs).
It utilizes microfluidic channels with inserted nodes to measure modulated current pulses generated by transiting cells.

Purpose of the Study:

To detail the application of NPS for sensitive detection and sizing of EVs.
To explain the use of NPS for mechanical phenotyping of cells by analyzing their deformability.

Main Methods:

NPS measures current pulse modulation as cells pass through a segmented microfluidic channel.
EV detection involves using NPS as a Coulter counter to detect size changes in antibody-bound colloids.
Cell mechanical phenotyping is achieved by analyzing cell passage through a constricted channel within the NPS setup.

Main Results:

NPS demonstrates exquisite sensitivity in detecting sub-micron size increases, suitable for EV quantification.
The technique allows for precise mechanical phenotyping by measuring cell squeezing through microfluidic constrictions.

Conclusions:

Node-Pore Sensing is a powerful and sensitive method for both EV detection and cell mechanical analysis.
The described NPS applications provide detailed protocols for researchers in cell biology and nanotechnology.