Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering Vascular Grafts: The Intersection of Manufacturing Techniques and Immune Response Modulation.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same author

Mitochondrial transplantation as an immunomodulatory strategy: Modulating polymorphonuclear leukocytes for functional tissue regeneration.

Cell transplantation·2026
Same author

Manuka honey and its component, methyl syringate, shift neutrophil release profiles from pro-inflammatory while preserving pro-regenerative growth factor release <i>in vitro</i>.

Frontiers in immunology·2026
Same author

Modeling heart rate patterns to quantify neonatal opioid withdrawal syndrome.

Pediatric research·2026
Same author

Utilizing High-Throughput Screening of dHL-60 Inflammatory Behaviors to Evaluate Honey-Derived Molecules' Potential as Immunomodulatory Biomaterial Additives.

Journal of inflammation research·2026
Same author

Immunomodulation and Mechanical Characterization of Manuka Honey-Incorporated Near-Field Electrospun Bioresorbable Vascular Grafts.

Bioengineering (Basel, Switzerland)·2025

Related Experiment Video

Updated: Oct 5, 2025

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
09:59

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

Published on: January 29, 2019

10.2K

Methods for Quantifying Neutrophil Extracellular Traps on Biomaterials.

Allison E Fetz1, William E King1,2, Benjamin A Minden-Birkenmaier1

  • 1Department of Biomedical Engineering, University of Memphis, Memphis, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 30, 2022
PubMed
Summary

This study presents two novel in vitro methods to quantify neutrophil extracellular traps (NETs) on biomaterials. These techniques, fluorescent imaging and ELISA, aid in understanding the host response to implanted materials.

Keywords:
BiomaterialsELISAElectrospun templateImage analysisNeutrophil extracellular traps (NETs)NeutrophilsQuantification

More Related Videos

Author Spotlight: Quantifying Neutrophil Extracellular Traps in Disease and Drug Screening Using Dual-Color Live-Cell Imaging
07:19

Author Spotlight: Quantifying Neutrophil Extracellular Traps in Disease and Drug Screening Using Dual-Color Live-Cell Imaging

Published on: December 1, 2023

1.2K
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
14:05

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

2.7K

Related Experiment Videos

Last Updated: Oct 5, 2025

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
09:59

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

Published on: January 29, 2019

10.2K
Author Spotlight: Quantifying Neutrophil Extracellular Traps in Disease and Drug Screening Using Dual-Color Live-Cell Imaging
07:19

Author Spotlight: Quantifying Neutrophil Extracellular Traps in Disease and Drug Screening Using Dual-Color Live-Cell Imaging

Published on: December 1, 2023

1.2K
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
14:05

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

2.7K

Area of Science:

  • Biomaterials Science
  • Immunology
  • Cell Biology

Background:

  • Neutrophils are key immune cells that accumulate at biomaterial sites, influencing tissue repair.
  • Neutrophil extracellular traps (NETs) are increasingly recognized for their role in the host response to biomaterials.
  • Quantifying NETs on biomaterial surfaces is crucial for understanding host-biomaterial interactions.

Purpose of the Study:

  • To describe and validate two distinct in vitro methods for quantifying NETs on biomaterial surfaces.
  • To provide high-throughput, low-bias techniques for NETs analysis.
  • To facilitate research into the host response to biomaterials.

Main Methods:

  • Development and optimization of a fluorescent image analysis procedure for NETs quantification.
  • Development and optimization of a NETs-based enzyme-linked immunosorbent assay (ELISA).
  • Validation of both methods using human neutrophils on electrospun polydioxanone templates and tissue culture plastic.

Main Results:

  • Both fluorescent image analysis and NETs-based ELISA provide reliable quantification of NETs on biomaterial surfaces.
  • Each method offers unique advantages and disadvantages, making them complementary.
  • The described methods are adaptable for large-scale applications and various biomaterial types.

Conclusions:

  • Established in vitro methods enable accurate quantification of NETs on biomaterials.
  • These techniques support the investigation of biomaterial-mediated inflammatory responses.
  • The adaptable protocols can be applied to diverse biomaterials for broader research applications.