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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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Related Experiment Video

Updated: Oct 5, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Development of a rapid detection method for genetically modified rice using the ultra-fast PCR system.

Min Ki Shin1,2, Seon Min Jeon1, Yong Eui Koo1

  • 1Food Safety Risk Assessment Department, National Institute of Food and Drug Safety Evaluation, Cheongju, 28159 Republic of Korea.

Food Science and Biotechnology
|January 31, 2022
PubMed
Summary

Rapid detection methods for unauthorized genetically modified (GM) rice events were developed using ultra-fast PCR. This technology offers a specific, sensitive, and reliable approach for monitoring GM rice in crops and products.

Keywords:
Event specific qualificationOryza sativaPlasmid referenceUnauthorized GM rice

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Area of Science:

  • Agricultural biotechnology
  • Molecular biology
  • Food safety

Background:

  • Genetically modified (GM) rice development includes traits for stress tolerance and pest resistance.
  • Unauthorized GM rice contamination incidents necessitate robust detection methods.
  • No GM rice is currently authorized for consumption in Korea, highlighting the need for safety management.

Purpose of the Study:

  • To develop rapid and reliable detection methods for five specific GM rice events (Bt63, KMD1, Kefeng6, Kefeng8, LLRice62).
  • To validate the applicability of ultra-fast PCR technology for GMO analysis in rice.

Main Methods:

  • Design and implementation of ultra-fast PCR assays for five GM rice events.
  • Conducting specificity, sensitivity, and applicability assays to confirm method performance.
  • Utilizing state-of-the-art ultra-fast PCR technology to decrease reaction times.

Main Results:

  • The developed ultra-fast PCR methods accurately identified the targeted GM rice events.
  • Assays confirmed the specificity, sensitivity, and reliability of the ultra-fast PCR system for GM rice detection.
  • The method demonstrated applicability for analyzing GM rice in both raw crops and processed products.

Conclusions:

  • Ultra-fast PCR is a specific, sensitive, and reliable tool for identifying and monitoring unauthorized GM rice events.
  • This technology provides a rapid and simple method for GMO analysis in various rice matrices.
  • The study serves as a reference for developing new analytical methods for unauthorized GMOs.