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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
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Visualization of a curated Oryza sativa L. CDPKs Protein-Protein Interaction Network (CDPK-OsPPIN)

Joana Marques1, Cleverson C Matiolli1, Isabel A Abreu1

  • 1Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Avenida da República, 2780-157 Oeiras, Portugal.

Micropublication Biology
|January 31, 2022
PubMed

Insights

Calcium-Dependent Protein Kinases (CDPKs) regulate plant growth and stress responses by phosphorylating proteins. This study introduces the Oryza sativa CDPK Protein-Protein Interaction Network (CDPK-OsPPIN) to explore CDPK functions and targets.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Calcium-Dependent Protein Kinases (CDPKs) are crucial regulators of plant physiology, mediating calcium ion (Ca²⁺) signals.
  • Understanding CDPK functions requires identifying their protein targets and roles in plant growth and stress responses.

Purpose of the Study:

  • To present a manually curated Protein-Protein Interaction Network for CDPKs in *Oryza sativa* (rice).
  • To provide a tool for researchers to investigate CDPKs' roles and functional diversity.

Main Methods:

  • Manual curation of protein-protein interaction data for *Oryza sativa* CDPKs.
  • Development of an interactive graphical network visualization tool (CDPK-OsPPIN).

Main Results:

  • The CDPK-OsPPIN database has been established.
  • The network facilitates the exploration of CDPK interactions and potential functions.

Conclusions:

  • The CDPK-OsPPIN serves as a valuable resource for plant scientists.
  • This network aids in generating hypotheses regarding CDPKs' involvement in plant stress and growth.

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