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Updated: Oct 5, 2025
Minimal Invasive Surgical Procedure of Inducing Myocardial Infarction in Mice
Published on: May 4, 2015
Noninvasively ventilated and easily operated mouse model of myocardial infarction
Yuan He1,2, Xinlin Lu3, Zheng Liang3
1Guangdong Provincial Engineering Technology Research Center for Molecular Diagnosis and Innovative Drugs Translation of Cardiopulmonary Vascular Diseases, University Joint Laboratory of Guangdong Province and Macao Region on Molecular Targets and Intervention of Cardiovascular Diseases, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.
Abstract:
The laboratory mouse is an animal model commonly used in cardiovascular research; however, it has technical challenges as a model for myocardial infarction (MI). In this study, a novel noninvasively ventilated and easily operated experimental mouse model of MI was established in Kunming mice based on a left anterior descending (LAD) coronary artery rupture. Overall, 95% of sham mice and 84% of mice with a ruptured LAD survived the surgery. The results of 2,3,5-triphenyl-2H-tetrazolium chloride and hematoxylin-eosin staining showed that obvious infarcts formed after LAD rupture. ST-segment elevation or depression emerging in the electrocardiogram of the novel MI model indicated a myocardial ischemic injury. Reduced cardiac contractility and increased cardiac troponin I and creatine kinase-MB after LAD-rupture implied myocardial necrosis. Furthermore, the serum levels of IL-1β and IL-6 were significantly upregulated after LAD-rupture. Overall, the LAD-rupture method, utilizing noninvasive ventilation, was a reliable and easily-performed model of MI in mice.
