Related Experiment Video
Updated: Oct 5, 2025
07:24
Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy
Published on: September 29, 2017
7.6K
Quantitative Methods to Assess Adipose Vasculature
Martina Rudnicki1, Alexandra Pislaru2, Tara L Haas3
1School of Kinesiology and Health Science, York University, Toronto, ON, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|January 31, 2022
Summary
Understanding adipose tissue vascularization is key for metabolic health. This study details methods to visualize and quantify these crucial capillary networks in adipose tissue.
Area of Science:
- Adipose tissue biology
- Vascular biology
- Cardiometabolic health
Background:
- Adipose tissue vascular networks are vital for tissue function and expansion.
- Capillary plasticity (angiogenesis/rarefaction) impacts cardiometabolic health.
- Quantifying adipose vascularization is essential for research.
Purpose of the Study:
- To provide an overview of methods for quantifying adipose vascularization.
- To detail protocols for visualizing and analyzing adipose vascular networks.
- To support research on factors regulating adipose tissue health.
Main Methods:
- Overview of techniques for adipose vascularization quantification.
- Protocols for whole-mount adipose tissue staining and imaging.
- Methods for paraffin-embedded adipose tissue section analysis.
- Quantitative analysis of vascularization from imaging data.
Main Results:
- Established protocols for visualizing adipose vascular structures.
- Provided methods for quantitative analysis of vascularization.
- Enabled detailed study of adipose tissue capillary networks.
Conclusions:
- Accurate visualization and quantification of adipose vascular networks are achievable.
- These methods are crucial for understanding adipose tissue health and cardiometabolic implications.
- The chapter offers practical guidance for researchers in the field.
